Head Shop the jars cool slowly. Leave them covered in the pan that was used
to sterilize them. Let them cool completely. The jars need to be at or
close to room temperature in order to inoculate. The spores will be
killed if the jars are not cool enough when they are inoculated. It will
take several hours to cool sufficiently. You may hear sounds as the jars
cool. This is normal.
Step 10:
Now comes the good part. Inoculation of the culture jars. Assuming
you have a viable, sterile spore syringe, you are now in a position to
inoculate the cultures and start the first phase of the growing cycle.
The needle of the spore syringe must be sterile. If your fingers or
anything other than the lid or contents of the culture jars comes in
contact with it, assume it is no longer sterile. If there is any doubt
about its condition, use a cigarette lighter to heat the entire needle.
Heat it until it glows red. Let it cool for a few minutes and squirt some
of the solution out of the syringe.
Shake the syringe. Make sure the spores are mixed well within the
syringe. This can be accomplished more easily if you pull the plunger
back on the syringe to get a little air into the syringe.
Remove the tin foil from each culture jar as you prepare to inoculate it.
Insert the needle of the syringe as far as it will go into a hole in the lid
of the culture jar and get the needle to press against the glass. Examine
the next figure for a simple diagram of how things should look. Inject
1/4 cc of solution at a site under each hole in the lid. Adaptation-4 A
total of 1 cc of solution for each jar. Adaptation-5
(5 of 29) [5/1/2002 6:54:17 PM
How To Grow Magic Mushrooms The ps from going in
the four holes in the lid while the jar is being sterilized. If you poked
your holes in the lid such that the sharp edges are pointing up, be
careful not to rip or puncture the tin foil. If you need to, you can add a
second or even a third piece of tin foil to make sure water will not drip
into the holes in the lid.
Step 8:
Now the culture jars need to be sterilized. Place the jars in a large
kitchen pot and add water so that water comes half way up the side of
the jars. Bring the water to a slow boil and place the lid on the pot.
From the time the water starts to boil, the jars need 1 hour to be
(4 of 29) 5/1/2002 6:54:17 PM
How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 2)
sterilized. Water should not be bubbling and splashing all over the
place. The jars should not be floating around in the water. The
substrate in the culture jars has the right amount of water in it already.
You do not want water leaking into the jars and changing the ratio. The
jars should not sit flat on the bottom of the pan. Too much heat can
transfer directly to the jars and cause a loss of moisture. You can place
a wash cloth inside the pan and set the jars directly on the wash cloth
to help prevent too much heat from transferring to the jars.
Step 9:
Let the jars cool slowly. Leave them covered in the pan that was used
to sterilize them. Let them cool completely. The jars need to be at or
close to room temperature in order to inoculate. The spores will be
killed if the jars are not cool enough when they are inoculated. It will
take several hours to cool sufficiently. You may hear sounds as the jars
cool. This is normal.
Step 10:
Now comes the good part. Inoculation of the culture jars. Assuming
you have a viable, sterile spore syringe, you are now in a position to
inoculate the cultures and start the first phase of the growing cycle.
The needle of the spore syringe must be sterile. If your fingers or
anything other than the lid or contents of the culture jars comes in
contact with it, assume it is no longer sterile. If there is any doubt
about its condition, use a cigarette lighter to heat the entire needle.
Heat it until it glows red. Let it cool for a few minutes and squirt some
of the solution out of the syringe.
Shake the syringe. Make sure the spores are mixed well within the
syringe. This can be accomplished more easily if you pull the plunger
back on the syringe to get a little air into the syringe.
Remove the tin foil from each culture jar as you prepare to inoculate it.
Insert the needle of the syringe as far as it will go into a hole in the lid
of the culture jar and get the needle to press against the glass. Examine
the next figure for a simple diagram of how things should look. Inject
1/4 cc of solution at a site under each hole in the lid. Adaptation-4 A
total of 1 cc of solution for each jar. Adaptation-5
(5 of 29) 5/1/2002 6:54:17 PM
How To Grow Magic Mushrooms The " />